Lady Bane (voiced by Tress MacNeille) is a recurring villain in the animated series. She is the evil witch character of the series and a love interest of the main villain, Duke Igthorn.
She is a tall and thin woman with long black hair hidden under hood. She always wears long robed clothing, has pale skin, purple eyeshadow and wears jewelry.
Lady Bane is in possession of a Gummi Medallion much like Zummi's, and craves any power she can get her hands on (including the Gummi Bears') for her bid for power. She is served by her jackal-like Troggles and, on occasion, Duke Igthorn, whose crush on her she finds extremely annoying, although she does agree to a dinner date with Igthorn if she figures it would forge an alliance to destroy the Gummis. In the episode "Rocking Chair Bear", it is discovered that Lady Bane is in reality extremely old, and uses her magic to "de-age" herself by stealing Sunni's youth until the other Gummis manage to reverse it. She has two friend-witches named Evelyn and Emmadrille.
- The creators of the television program took inspiration for the character by the historical figure of Lucrezia Borgia.
- Lady Bane's appearance and personality seems to be based on The Evil Queen from Snow White and the Seven Dwarfs.
- In her final appearance, she is stated to be a hundred-years-old, which is rare in humans, but has happened.